硅纳米颗粒的栅栏发光成像
Gated Luminescence Imaging of Silicon Nanoparticles
作者:Jinmyoung Joo;Xiangyou Liu;Venkata Ramana Kotamraju;Erkki Ruoslahti;Yoonkey Nam;Michael J. Sailor;
关键词:time-gated luminescence imaging,bioimaging,intravital imaging,targeting peptides,tumor,cancer,in vivoimaging,iRGD,Show More
DOI:https://doi.org/10.1021/acsnano.5b01594
发表时间:2015年
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摘要
纳米晶硅的发光寿命通常在微秒量级上,比细胞和组织中自然存在的荧光分子表现出的纳秒寿命明显更长。时间门控成像,其中图像是在激发脉冲终止后一段时间获取的,允许区分硅纳米颗粒探针和内源信号。由于硅发射的微秒时间尺度,时间门控成像对于这种生物相容性和无毒性探针相对简单实现。文章描述了一个具有约10纳秒分辨率的时间门控系统,采用强化的CCD相机和脉冲LED或激光激发源。该方法通过追踪含有靶向肿瘤肽iRGD的介孔硅纳米颗粒在活体小鼠的视网膜注射后的命运进行了演示。由于靶向组织中探针浓度较低并且组织自发荧光背景信号相对较高,体内系统给药的纳米颗粒成像尤其具有挑战性。靶向组织中对比度改进超过100倍(相对于稳态成像)得到了证实。
Abstract
The luminescence lifetime of nanocrystalline silicon is typically on the order of microseconds, significantly longer than the nanosecond lifetimes exhibited by fluorescent molecules naturally present in cells and tissues. Time-gated imaging, where the image is acquired at a time after termination of an excitation pulse, allows discrimination of a silicon nanoparticle probe from these endogenous signals. Because of the microsecond time scale for silicon emission, time-gated imaging is relatively simple to implement for this biocompatible and nontoxic probe. Here a time-gated system with ∼10 ns resolution is described, using an intensified CCD camera and pulsed LED or laser excitation sources. The method is demonstrated by tracking the fate of mesoporous silicon nanoparticles containing the tumor-targeting peptide iRGD, administered by retro-orbital injection into live mice. Imaging of such systemically administered nanoparticles in vivo is particularly challenging because of the low concentration of probe in the targeted tissues and relatively high background signals from tissue autofluorescence. Contrast improvements of >100-fold (relative to steady-state imaging) is demonstrated in the targeted tissues.
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