膜辅助生长DNA原子级纳米结构阵列
Membrane-Assisted Growth of DNA Origami Nanostructure Arrays
作者:Samet Kocabey;Susanne Kempter;Jonathan List;Yongzheng Xing;Wooli Bae;Daniel Schiffels;William M. Shih;Friedrich C. Simmel;Tim Liedl;
关键词:DNA origami,DNA nanotechnology,lipid membrane,diffusion,arrays,cholesterol,clathrin
DOI:https://doi.org/10.1021/acsnano.5b00161
发表时间:2015年
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摘要
生物膜在生物体内扮演着许多重要任务。除了分隔细胞体积外,膜将膜相关蛋白质的空间限制在了二维平面上,极大地增加了它们相互作用并组装成多蛋白复合物的可能性。在这项研究中,我们使用了两种功能化了胆固醇基团作为膜锚的DNA原子结构——一个三层的矩形块和一个Y形的DNA结构——来模拟膜辅助组装形成分层超结构的过程,这个过程发生在支持脂质双分子层和小一层脂质泡囊上。设计上,DNA结构依靠胆固醇锚固定在脂质双分子层上,并在二维空间中自由扩散,其扩散系数取决于其大小和胆固醇修饰的数量。加入到双分子层上的原子结构的寡聚核苷酸集合能够诱导线性高聚物或二维晶格的生长。Y形的DNA原子结构组装成三翼同聚体,进一步组装成六角形和五角形的弱有序阵列,类似于覆盖有凯氏网的小窝体的几何结构。我们的研究结果展示了实现人工自组装系统的潜力,这些系统能够模拟细胞质膜上多面体晶格的分级组装形式。
Abstract
Biological membranes fulfill many important tasks within living organisms. In addition to separating cellular volumes, membranes confine the space available to membrane-associated proteins to two dimensions (2D), which greatly increases their probability to interact with each other and assemble into multiprotein complexes. We here employed two DNA origami structures functionalized with cholesterol moieties as membrane anchors—a three-layered rectangular block and a Y-shaped DNA structure—to mimic membrane-assisted assembly into hierarchical superstructures on supported lipid bilayers and small unilamellar vesicles. As designed, the DNA constructs adhered to the lipid bilayers mediated by the cholesterol anchors and diffused freely in 2D with diffusion coefficients depending on their size and number of cholesterol modifications. Different sets of multimerization oligonucleotides added to bilayer-bound origami block structures induced the growth of either linear polymers or two-dimensional lattices on the membrane. Y-shaped DNA origami structures associated into triskelion homotrimers and further assembled into weakly ordered arrays of hexagons and pentagons, which resembled the geometry of clathrin-coated pits. Our results demonstrate the potential to realize artificial self-assembling systems that mimic the hierarchical formation of polyhedral lattices on cytoplasmic membranes.
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