毛细管流逐层: 一种微流控平台,用于高通量组装和筛选纳米层膜库
Capillary Flow Layer-by-Layer: A Microfluidic Platform for the High-Throughput Assembly and Screening of Nanolayered Film Libraries
作者:Steven A. Castleberry;Wei Li;Di Deng;Sarah Mayner;Paula T. Hammond;
关键词:layer-by-layer,LbL,high-throughput,polyelectrolyte multilayers,PEM,screening,ultrathin films
DOI:https://doi.org/10.1021/nn501963q
发表时间:2014年
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摘要
逐层组装(LbL)是一种强大的工具,在能源、生物材料、活性表面和膜等现实应用中越来越多见;然而,目前的技术要求使用大量材料进行单独样品构建。在这里,我们描述了一种利用微流控装置内的毛细流来驱动LbL薄膜库的高通量组装的技术。这种毛细流逐层组装(CF-LbL)方法显著减少了材料浪费,提高了质量控制,并将LbL的潜在应用扩展到新的研究领域。这种方法可以作为一个简单的实验室台面装置使用,也可以与液体处理的机器人结合起来扩大库的规模。在这里,我们描述并展示了这种技术,并确立了它重新创造和扩展薄膜生长和形态学已知文献的能力。我们使用相同的平台来评估细胞粘附和增殖等生物特性,最终提供了这种方法用于识别用于常用细胞类型表面DNA转染的LbL薄膜的示例。
Abstract
Layer-by-layer (LbL) assembly is a powerful tool with increasing real world applications in energy, biomaterials, active surfaces, and membranes; however, the current state of the art requires individual sample construction using large quantities of material. Here we describe a technique using capillary flow within a microfluidic device to drive high-throughput assembly of LbL film libraries. This capillary flow layer-by-layer (CF-LbL) method significantly reduces material waste, improves quality control, and expands the potential applications of LbL into new research spaces. The method can be operated as a simple lab benchtop apparatus or combined with liquid-handling robotics to extend the library size. Here we describe and demonstrate the technique and establish its ability to recreate and expand on the known literature for film growth and morphology. We use the same platform to assay biological properties such as cell adhesion and proliferation and ultimately provide an example of the use of this approach to identify LbL films for surface-based DNA transfection of commonly used cell types.
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