核膜样高分子泡泡的制备和机制洞察,用于便捷装载生物大分子和增强生物催化活性
Preparation and Mechanism Insight of Nuclear Envelope-like Polymer Vesicles for Facile Loading of Biomacromolecules and Enhanced Biocatalytic Activity
作者:Yunqing Zhu;Fangyingkai Wang;Cong Zhang;Jianzhong Du;
关键词:polymer vesicles,lateral segregation mechanism,nuclear envelope,biomacromolecule encapsulation,biocatalysis
DOI:https://doi.org/10.1021/nn502386j
发表时间:2014年
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摘要
敏感且脆弱的生物大分子(例如葡萄糖氧化酶,血红蛋白和核糖核酸(RNA))在纯水介质中通过合成载体的方便装载是一个重要的技术挑战。受连接细胞核和细胞质的核孔复合物启发,我们在此描述了一种聚合物核膜样囊泡(NEV)的发展,以解决这个问题。NEV经过量身定制,形成了基于纳米相分离的聚合物孔复合物(70纳米,类似于核孔复合物)在囊泡膜中,在自组装过程中通过荧光光谱和动态光散射(DLS)进行了确认。这种pH触发的聚合物孔复合物可以介导生物大分子穿越囊泡膜。此外,NEV通过H 海绵效应有助于促进天然的连续酶催化反应。这种简单的策略也可以扩展到模拟其他合成细胞器。
Abstract
The facile loading of sensitive and fragile biomacromolecules, such as glucose oxidase, hemoglobin, and ribonucleic acid (RNA), via synthetic vehicles directly in pure aqueous media is an important technical challenge. Inspired by the nucleus pore complex that connects the cell nucleus and the cytoplasm across the nuclear envelope, here we describe the development of a kind of polymeric nuclear envelope-like vesicle (NEV) to address this problem. The NEV is tailored to form the polymer pore complex (70 nm, similar to a nucleus pore complex) within the vesicle membrane based on nanophase segregation, which is confirmed via fluorescence spectrometry and dynamic light scattering (DLS) during self-assembly. This pH-triggered polymer pore complex can mediate the transportation of biomacromolecules across the vesicle membrane. Moreover, the NEVs facilitate the natural consecutive enzyme-catalyzed reactions via the H+ sponge effect. This simple strategy might also be extended for mimicking other synthetic cell organelles.
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