用于体内对比增强磁共振成像的生物相容性肽包被超小型超顺磁性氧化铁纳米颗粒
Biocompatible Peptide-Coated Ultrasmall Superparamagnetic Iron Oxide Nanoparticles forIn VivoContrast-Enhanced Magnetic Resonance Imaging
作者:Heng Li Chee;Ching Ruey R. Gan;Michael Ng;Lionel Low;David G. Fernig;Kishore K. Bhakoo;David Paramelle;
关键词:peptide,coating,iron oxide nanoparticles,biocompatibility,magnetic resonance imaging
DOI:https://doi.org/10.1021/acsnano.7b07572
发表时间:2018年
- 文献详情
- 相似文献
摘要
体内对比增强磁共振成像 (MRI) 试剂的生物相容性和性能对于其转化为临床至关重要。基于纳米颗粒的 MRI 造影剂(例如超小超顺磁性氧化铁纳米颗粒 (USPION))的表面涂层质量对于确保生物环境中的高胶体稳定性、改善的磁性能以及在循环液和组织中的分散至关重要。在此,我们报告了 21 种肽和配体库的设计,并鉴定了 USPION 表面高度稳定的自组装单层。使用几个严格的标准制备和选择了总共 86 种不同的肽包被的 USPION,例如生理条件下电解质诱导聚集的稳定性、防止与细胞非特异性结合、不存在细胞毒性和对比增强的体内 MRI。双磷酸化肽 2PG-S*VVVT-PEG4-ol 为 USPION 提供最高的生物相容性和性能,没有可检测到的毒性或对活细胞的粘附。 2PG-S*VVVT-PEG4-ol 包被的 USPION 显示出增强的磁共振特性,r1 (2.4 mM–1·s–1) 和 r2 (217.8 mM–1·s–1) 弛豫率,以及更大的 r2/与市售 MRI 造影剂相比,r1 弛豫率 (>90)。此外,我们还证明了 2PG-S*VVVT-PEG4-ol 涂层 USPION 作为 T2 造影剂在体内 MRI 应用中的实用性。实现了肝脏的高对比度增强以及肝脏肿瘤的检测,显着改善了肿瘤与肝脏对比度的对比噪声比。据设想,所报道的肽包被的 USPION 有可能实现肿瘤的特异性靶向,从而通过 MRI 早期检测癌症。
Abstract
The biocompatibility and performance of reagents for in vivo contrast-enhanced magnetic resonance imaging (MRI) are essential for their translation to the clinic. The quality of the surface coating of nanoparticle-based MRI contrast agents, such as ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), is critical to ensure high colloidal stability in biological environments, improved magnetic performance, and dispersion in circulatory fluids and tissues. Herein, we report the design of a library of 21 peptides and ligands and identify highly stable self-assembled monolayers on the USPIONs’ surface. A total of 86 different peptide-coated USPIONs are prepared and selected using several stringent criteria, such as stability against electrolyte-induced aggregation in physiological conditions, prevention of nonspecific binding to cells, and absence of cellular toxicity and contrast-enhanced in vivo MRI. The bisphosphorylated peptide 2PG-S*VVVT-PEG4-ol provides the highest biocompatibility and performance for USPIONs, with no detectable toxicity or adhesion to live cells. The 2PG-S*VVVT-PEG4-ol-coated USPIONs show enhanced magnetic resonance properties, r1 (2.4 mM–1·s–1) and r2 (217.8 mM–1·s–1) relaxivities, and greater r2/r1 relaxivity ratios (>90) when compared to those of commercially available MRI contrast agents. Furthermore, we demonstrate the utility of 2PG-S*VVVT-PEG4-ol-coated USPIONs as a T2 contrast agent for in vivo MRI applications. High contrast enhancement of the liver is achieved as well as detection of liver tumors, with significant improvement of the contrast-to-noise ratio of tumor-to-liver contrast. It is envisaged that the reported peptide-coated USPIONs have the potential to allow for the specific targeting of tumors and hence early detection of cancer by MRI.
-
Ultrafast Interlayer Charge Transfer Outcompeting Intralayer Valley Relaxation in Few-Layer 2D Heterostructures
引用量:0发表时间:2023年
-
Vanadium Carbide Nanosheets with Broad-Spectrum Antioxidant Activity for Pulmonary Fibrosis Therapy
引用量:0发表时间:2023年
-
“Reaction”-Like Shaping of Self-Delivery Supramolecular Nanodrugs in the Nanoprecipitation Process
引用量:0发表时间:2023年
-
Vanadium Intercalation into Niobium Disulfide to Enhance the Catalytic Activity for Lithium–Sulfur Batteries
引用量:0发表时间:2023年
-
Mapping Antibody Domain Exposure on Nanoparticle Surfaces Using DNA-PAINT
引用量:0发表时间:2023年
-
Development of Multifunctional Nanoencapsulatedtrans-Resveratrol/Chitosan Nutraceutical Edible Coating for Strawberry Preservation
引用量:0发表时间:2023年
-
Solution-Processed Flexible Transparent Electrodes for Printable Electronics
引用量:0发表时间:2023年
-
Amorphizing Metal Selenides-Based ROS Biocatalysts at Surface Nanolayer toward Ultrafast Inflammatory Diabetic Wound Healing
引用量:0发表时间:2023年
-
Direct Imaging of “Patch-Clasping” and Relaxation in Robust and Flexible Nanoparticle Assemblies
引用量:0发表时间:2023年
-
An Integrated Multilevel Approach Unveils Complex Seed–Nanoparticle Interactions and Their Implications for Seed Priming
引用量:0发表时间:2023年