Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulos
Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein–carbohydrate interactions both in endogenous recognition systems and pathogen–host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques – from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins.
- Inositol 1,4,5-Trisphosphate Mass Assay
- Analysis of Yeast Artificial Chromosome Clones
- 盆栽果树(fruit culture in pot)
- Capillary Electrophoresis in Clinical Analysis
- Sample Preparation for SFM Imaging of DNA, Proteins, and DNAProtein Complexes
- 肝脏在糖代谢中的作用
- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay
- 桃奴(pygmy fruit of peach)
- 人前降钙素(PCT)酶联免疫分析(ELISA)
- Enzyme-Labeled DNA Probes