PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples
Platelet transcriptome studies provide a powerful tool in the analysis of disorders affecting the megakaryocytic-platelet lineage. However, individualised platelet gene expression profiling is hampered by the exceptionally low yield of platelet mRNA. The yield of mRNA transcripts that can be obtained from small-scale platelet preparations is generally not sufficient for standard RNA-labeling reactions used in expression profiling. Furthermore, leukocyte contaminants in platelet preparations are a potential source of ‘unwanted’ mRNA since they contain several orders of magnitude more mRNA than platelets. To overcome these limitations a strategy that combines leukocyte filtration and a PCR-based amplification technique (SMART™TM) has been developed and extensively evaluated.
- GCG: Assembly of Sequences into New Sequence Constructs
- Chromatography-Based Purification of Adeno-Associated Virus
- A Protocol for VIGS in Arabidopsis Thaliana Using a One-Step TYMV-Derived Vector
- Analysis of Allele-Specific Gene Expression
- Construction of cDNA Libraries
- Cloning and Detection of HIV-1-Encoded MicroRNA
- Incorporation of Gene Ontology Annotations to Enhance Microarray Data Analysis
- The Use of Robotic Workstations in DNA Sequencing
- Heterologous Protein Expression by Lactococcus lactis
- Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors