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基因表达差异显示实验>Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes
Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes
关键词： preparing lambda libraries来源： 互联网
Complementary DNA (cDNA) libraries represent the information encoded in the messenger RNA (mRNA) of a particular tissue or organism. RNA molecules are exceptionally labile and difficult to amplify in their natural form. For this reason, a cDNA library is created by converting the information encoded by the RNA into a stable DNA duplex (cDNA) and then inserting it into a self-replicating lambda or plasmid vector. Individual cDNA representing the original genetic information can be isolated from the cDNA library and examined with relative ease.
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