Electrophoretic Karyotype Analysis Pulsed Field Gel Electrophoresis
Electrophoretic karyotyping is a term first introduced by Carle and Olson in 1985 (1 ) to describe the use of the new technique of pulsed field gel electrophoresis (PFGE) to visualize whole chromosomes from unicellular organisms. Conventional agarose gel electrophoresis has a useful upper size limit of about 20 kilobases (kb). PFGE extends this limit to at least 5.7 megabases (Mb) (2 ) and may be up to 12 Mb (3 ), encompassing the size range of chromosomes from many bacteria, fungi, and protozoa. Chromosomes from most of these organisms fail to condense sufficiently during mitosis to allow karotyping by light microscopy and, prior to the advent of PFGE, estimates of genome size and chromosome number were based on genetic linkage analysis, DNA reassociation kinetics, and in some cases, electron microscopy.
- Multi-SNP Haplotype Analysis Methods for Association Analysis
- Quantitative Real-Time PCR in aDNA Research
- RNAi-Inducing Lentiviral Vectors for Anti-HIV-1 Gene Therapy
- Analysis of Methylated Circulating DNA in Cancer Patients’ Blood
- Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila
- Novel Approach for the Development of New Antibodies Directed Against Transposase-Derived Proteins Encoded by Human Neogenes
- Epistemological Impacts of Horizontal Gene Transfer on Classification in Microbiology
- Gene Mapping Using 3H-Labeled Heterologous Probes
- Lentiviral Overexpression of miRNAs
- Generation and PCR Screening of Bacteriophage Sublibraries Enriched for Rare Clones (the Sublibrary Method)