Expressed Sequence Tags: An Overview
Expressed sequence tags (ESTs) are fragments of mRNA sequences derived through single sequencing reactions performed on randomly selected clones from cDNA libraries. To date, over 45 million ESTs have been generated from over 1400 different species of eukaryotes. For the most part, EST projects are used to either complement existing genome projects or serve as low-cost alternatives for purposes of gene discovery. However, with improvements in accuracy and coverage, they are beginning to find application in fields such as phylogenetics, transcript profiling and proteomics. This volume provides practical details on the generation and analysis of ESTs. Chapters are presented which cover creation of cDNA libraries; generation and processing of sequence data; bioinformatics analysis of ESTs; and their application to phylogenetics and transcript profiling.
- Use of Defective Herpes-Derived Plasmid Vectors
- High Resolution MICA Genotyping by Sequence-Based Typing (SBT)
- Immunohistochemical Analysis of Transgene Expression
- Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis
- Fluorescence in situ Hybridization (FISH) on Tissue Cryosections
- siRNA Delivery via Electropulsation: A Review of the Basic Processes
- Quantitative Analysis of RNA Modifications
- The Use of Polymerase Chain Reaction for Chromosome Assignment
- Size Analysis of Polyglutamine Protein Aggregates Using Fluorescence Detection in an Analytical Ultracentrifuge
- Analysis of Transgenic Mice