Molecular Genetics of DNA Viruses Recombinant Virus Technology
Recombinant viral genomes cloned onto BAC vectors can be subjected to extensive molecula genetic analysis in the context of E. coli. Thus, the recombinant virus technology exploits the power of prokaryotic genetics to introduce all kinds of mutations into the recombinant genome. All available techniques are based on homologous recombination between a targeting vector carrying the mutated version of the gene of interest and the recombinant virus. After modification, the mutant viral genome is stably introduced into eukaryotic cells permissive for viral lytic replication. In these cells, mutant viral genomes can be packaged into infectious particles to evaluate the effect of these mutations in the context of the complete genome.
- Small- to Large-Scale Production of Lentivirus Vectors
- Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-Dimensional (2D) NativeNative
- Genotyping of Single Nucleotide Polymorphisms by Arrayed Primer Extension
- Detection of Epigenetic Alterations Using Tiling Arrays
- PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones
- How Do You Find Transcription Factors Computational Approaches to Compile and Annotate Repertoires of Regulators for Any Genome
- Molecular Assays to Investigate Chromatin Changes During DNA Double-Strand Break Repair in Yeast
- Methods to Study the Role of Sirtuins in Genome Stability
- Studying DNA Replication Fork Stability in Xenopus Egg Extract
- Application of DNA Microarray for Screening Metagenome Library Clones