Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein
Fragile X syndrome, a common form of inherited mental retardation, is caused by the loss of the Fragile X mental retardation protein (FMRP). FMRP, which may regulate translation in neurons, not only associates with specific messenger RNAs (mRNAs) and with microRNAs (miRNAs), but also associates with the components of the miRNA pathway, including the Dicer and Argonaute proteins. It has been proposed that FMRP regulates the translation of its mRNA targets through miRNAs. In this chapter, we describe the protocol to identify the mRNAs and miRNAs associated with FMRP in vivo. The same method could also be applied to other RNA-binding proteins interacting with specific mRNAs or miRNAs.
- In-Line Probing Analysis of Riboswitches
- Poly(T) Adaptor RT-PCR
- Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes
- Application of Pulsed-Field Gel Electrophoresis to Genetic Diagnosis
- Design of Small Interfering RNAs for Antiviral Applications
- Using Pyrosequencing to Measure Allele-Specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences
- Gene Delivery Using Physical Methods: An Overview
- Reporter Genes
- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis
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