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基因表达差异显示实验>Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis: Versatile Strategy to Move Beyond Structural Limit
Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis: Versatile Strategy to Move Beyond Structural Limit
关键词： combining site-specific site specific chemical modification site-directed site directed mutagenesis versatile strategy move beyo来源： 互联网
The invention of site-directed amino acid (AA) mutagenesis (1) has revolutionized the way in which enzyme mechanism and specificity can be probed, while simultaneously providing a route for engineering novel enzyme properties. Prior to this very precise method of AA replacement, protein chemists utilized chemical modification and chemical mutagenesis techniques to alter protein and enzyme properties (2–4).
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