Direct Sequencing of Polymerase Chain Reaction Products
The advantages of direct sequencing of polymerase chain reaction (PCR) products over conventional sequencing of cloned, single-stranded DNA are manifold. Speed is perhaps the greatest asset. Time-consuming creation and screening of libraries, fragment purification, subcloning, bacterial transfection, and plasmid preparation steps are all eliminated. Cost is an advantage for similar reasons. The ability to sequence thousands or even millions of different templates at once, thereby obtaining pooled averages of mutated or polymorphic sequences, is another benefit. Moreover, short DNA sequences not obtainable by conventional cloning, such as DNA from paraffin-embedded tissues, can be sequenced using PCR sequencing.
- Fast Micromethod DNA Single-Strand-Break Assay
- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
- Generating Conditional Knockout Mice
- Molecular Mapping and Breeding with Microsatellite Markers
- Chromatin Immunoprecipitation Assay of Piwi in Drosophila
- Isolation and Analysis of DNA Derived from Nucleosome-Free Regions
- Detection of Chimerism in YAC Clones
- Simultaneous Ultrasensitive Subpopulation Staining/Hybridization In Situ (SUSHI) in HIV-1 Disease Monitoring
- Diethyl Pyrocarbonate as a Probe of Protein-DNA Interactions
- In Vivo Analysis of RNA Editing in Plastids