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基因表达差异显示实验>Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography
Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography
关键词: analysis polymerase chain reaction来源: 互联网
Denaturing high-performance liquid chromatography (DHPLC) analysis is an ionpair reversed-phase high performance liquid chromatography for performing analytical separations of DNA based on temperature: analysis is performed at a temperature sufficient to partially denature DNA heteroduplexes. The technology detects single-base changes as efficiently as short deletions and insertions. The chance that a mutation cannot be detected is 0.5%.
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