Cytochrome P450-Based Gene Therapies for Cancer
Cytochrome P450 (CYP) is comprosed of a family of hemeprotein monooxygenases that catalyze reactions as diverse as the biosynthesis of steroid hormones, metabolism of fat-soluble vitamins, oxidation of unsaturated fatty acids, and metabolism of drugs, pollutants, and other xenobiotics (for a review, see ref. 1 ). About 55 CYP genes, grouped into 17 gene families, are present in the human genome. CYPs belonging to gene families 1, 2, and 3 are particularly active in drug and xenobiotic metabolism and are most abundantly expressed in the liver and other tissues that come in contact with foreign chemicals. Large interindividual variations in CYP expression and, consequently, CYP-dependent drug metabolism are seen in humans, as a result of both genetic and environmental factors.
- Integrative Analysis of ChIP-Chip and ChIP-Seq Dataset
- Using the Semi-synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1
- Isolation and Profiling of Protein-Associated Small RNAs
- Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
- Using the Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples
- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction
- Generation of Transgenic Animals by Use of YACs
- Isolation and Maintenance of Primate Embryonic Stem Cells
- Heterologous Gene Expression by Chromosomal Integration in Fission Yeast
- Web Tools for the Prioritization of Candidate Disease Genes