Primed In Situ (PRINS) Labeling of DNA
During the last two decades, techniques for the detection of specific DNA and RNA sequences in situ have been developed. In the beginning, only radioactive detection was possible, which to some extent made these techniques unsuited for routine purposes. Within the last decade, a number of nonradioactive detection methods have become available, resulting in a much more widespread use of in situ hybridization. These methods have become of great value, e.g., as a supplement to traditional cytogenetic analysis in the identification and quantification of numerical and/or structural aberrations in both clinical genetics and cancer diagnosis.
- En Passant Mutagenesis: A Two Step Markerless Red Recombination System
- Combined Bisulfite Restriction Analysis (COBRA)
- Combined Chromatin Immunoprecipitation and Bisulfite Methylation Sequencing Analysis
- Staden: Searching for Motifs in Protein Sequences
- Technological Issues and Experimental Design of Gene Association Studies
- Transgenic Studies in the Mouse: Improving the Technology Towards a Conditional Temporal and Spatial Approach
- How Do You Find Transcription Factors Computational Approaches to Compile and Annotate Repertoires of Regulators for Any Genome
- A PhenolChloroform Protocol for Extracting DNA from Ancient Samples
- Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection
- Web Tools for the Prioritization of Candidate Disease Genes