Expression Profiling of microRNAs in Cancer Cells: Technical Considerations
MicroRNAs (miRNAs) represent a class of small, noncoding RNAs. These small RNAs are involved in diverse biological processes, and it has been predicted that about one third of human messenger RNAs (mRNAs) appear to be miRNA targets, underlying the major influence of miRNAs on almost all cellular pathways. Deviation from the normal pattern of miRNA expression has been implicated in several diseases. Among human diseases, it has been shown that changes in miRNA expression correlate with various human cancers. Thus, miRNA profiling could contribute to more precise tumor classification and better prediction of the therapeutic outcome. This chapter summarizes these recent findings and highlights the technical advances in miRNA probe preparation, miRNA expression profiling and target identification.
- 16S rRNA Targeted DGGE Fingerprinting of Microbial Communities
- Manipulating Large Insert Clones for Transgenesis
- Using Arabidopsis-Related Model Species (ARMS): Growth, Genetic Transformation, and Comparative Genomics
- Cryopreservation of Transgenic Sheep Lines
- Insights on Imprinting from Beyond Mice and Men
- Direct Sequencing of DNA Produced in a Polymerase Chain Reaction
- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro
- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate
- Scanning Probe and Nanopore DNA Sequencing: Core Techniques and Possibilities
- Functional Studies of DNAProtein Interactions Using FRET Techniques