Electrochemical DNA Biosensors: Protocols for Intercalator-Based Detection of Hybridization in Solution and at the Surface
An electrochemical DNA biosensor is a device that utilizes the inherent ability of two complementary strands of nucleic acids to form a double helix. The specificity of this reaction, namely hybridization, is used in the detection of target DNA sequences with a view toward developing point-of-care devices. Since the early 1990s, great progress has been made in this field, but there are still numerous challenges to overcome. This chapter describes the components of an electrochemical DNA biosensor for researchers new to the field, paying particular attention to intercalator-based DNA biosensors. We will use a well-defined electro-active DNA intercalator Hoechst 33258, as our running example. Two of the most classic DNA sensing methods: solution-based and surface-immobilized methods are discussed, along with guiding notes that would help identify and overcome possible problems in a typical electrochemical DNA biosensor experiment.
- Ensemble and Single-Molecule Detected Time-Resolved FRET Methods in Studies of Protein Conformations and Dynamics
- Three-Dimensional Analysis of Single Particles by Electron Microscopy: Sample Preparation and Data Acquisition
- Fractal Characterization of Complexity in Dynamic Signals: Application to Cerebral Hemodynamics
- Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells
- Elemental Mapping by Electron Energy Loss Spectroscopy in Biology
- Design and Characterization of Magnetic Resonance Imaging Gene Reporters
- Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Store
- Imaging Fate of Stem Cells at a Cellular Resolution in the Brains of Mice
- MRI in Experimental Stroke
- 利用荧光光学投影断层成像技术进行全心脏成像的标准化过程