In Vivo Imaging of Oligonucleotide Delivery
RNA interference (RNAi) has rapidly become a powerful tool for drug-target discovery and therapeutics. Cancer is an important application for RNAi therapeutics, since abnormal gene regulation is thought to contribute to the pathogenesis and maintenance of the metastatic phenotype of cancer. Many oncogenic genes present enticing therapeutic target possibilities for RNAi. Small interfering RNA (siRNA) and microRNA (miRNA) are potent and specific examples of RNAi are able to silence tumor-related genes and multiple oncogenic pathways and appear to be a rational approach to inhibit tumor growth. In subsequent in vivo studies, an appropriate animal model must be developed for a better evaluation of gene-silencing effects on tumors. How to evaluate the effect of siRNA and miRNA in an in vivo therapeutic model is also important. Bioluminescence imaging is an optical imaging method that can evaluate RNAi in vivo.
- Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS
- Intravital Fluorescence Microscopic Molecular Imaging of Atherosclerosis
- Imaging and Analysis of Three-Dimensional Cell Culture Models
- Magnetic Resonance Microscopy of Mouse Brain Development
- Imaging of Cellular Oxygen and Analysis of Metabolic Responses of Mammalian Cells
- Elemental and Isotopic Imaging of Biological Samples Using NanoSIMS
- Age Estimation Using Tooth Cementum Annulation
- Single mRNP Tracking in Living Mammalian Cells
- 荧光显微镜原理
- 近红外手术“导航”的应用