Automated Discovery of Single Nucleotide Polymorphism and Simple Sequence Repeat Molecular Genetic Markers
Molecular genetic markers represent one of the most powerful tools for the analysis of genomes. Molecular marker technology has developed rapidly over the last decade, and two forms of sequence-based markers, simple sequence repeats (SSRs), also known as microsatellites, and single nucleotide polymorphisms (SNPs), now predominate applications in modern genetic analysis. The availability of large sequence data sets permits mining for SSRs and SNPs, which may then be applied to genetic trait mapping and marker-assisted selection. Here, we describe Web-based automated methods for the discovery of these SSRs and SNPs from sequence data. SSRPrimer enables the real-time discovery of SSRs within submitted DNA sequences, with the concomitant design of PCR primers for SSR amplification. Alternatively, users may browse the SSR Taxonomy Tree to identify predetermined SSR amplification primers for any species represented within the GenBank database. SNPServer uses a redundancy-based approach to identify SNPs within DNA sequence data. Following submission of a sequence of interest, SNPServer uses BLAST to identify similar sequences, CAP3 to cluster and assemble these sequences, and then the SNP discovery software autoSNP to detect SNPs and insertion/deletion (indel) polymorphisms.
- 光合作用基础知识讲座系列二:叶绿体和光合色素
- 植物维生素C含量的测定
- 阴地植物
- 观音座莲目(Marattiales)
- Genetic Engineering of Novel Flower Colors in Floricultural Plants: Recent Advances via Transgenic Approaches
- Bimolecular-Fluorescence Complementation Assay to Monitor KinaseSubstrate Interactions In Vivo
- Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
- Chemical Identification Strategies Using Liquid Chromatography-Photodiode Array-Solid-Phase Extraction-Nuclear Magnetic Resonanc
- Instrumentation
- Use of Molecular Methods for the Detection of Fungal Spores