Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry
Mass spectrometry (MS) offers a sensitive, reliable, and highly accurate method for measurement of isotopic labeling, which is required for generating comprehensive flux maps using metabolic flux analysis (MFA). We present protocols for assessing isotope labeling in a wide range of biochemical species, including proteinogenic amino acids, free organic and amino acids, sugar phosphates, lipids, starch-glucose, and RNA-ribose. We describe the steps of sample preparation, MS analysis, and data handling required to obtain high-quality isotope labeling measurements that are applicable to MFA. By selecting target analytes that maximize identifiability of the key fluxes of interest, MS measurements of isotope labeling can provide a powerful platform for assessing metabolic fluxes in complex biochemical networks.
- 菊目
- 植物人工繁殖技术
- 鞭枝藻属(Mastigocladus)
- 花粉管
- 榆叶梅 Prunus triloba
- 白蜡树
- Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants
- Indirect Immunofluorescence Microscopy for the Detection and Identification of Plant Pathogenic Bacteria (In Particular for Rals
- Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength
- Eucalyptus