Peroxisome Isolation
关键词: peroxisome isolation来源: 互联网
The group of De Duve first described the isolation of peroxisomes from rat liver tissue. These organelles can be separated from other sub-cellular organelles, such as lysosomes and mitochondria, because of their relatively high equilibrium density in sucrose (approx 1.24 g/cm3 ). The isolation of peroxisomes from the yeast Saccharomyces cerevisiae , however, has been hampered by the fact that under standard growth conditions peroxisomes are present in low numbers. Also, the lability of peroxisomes in general has complicated their purification.
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