Innate Immune Receptors for Nucleic Acids
The innate immune system has evolved to detect microbes and sterile tissue damage with the help of a series of signaling receptors. One key strategy is to detect infectious microbes or host cell damage by recognizing nucleic acids that are modified or appear in compartment normally devoid of nucleic acids. Here, we describe two methods that allow studying the molecular interaction between various nucleic acid recognizing signaling receptors with their ligands. A ligand pull-down assay can be used to show a known interaction between a ligand and its receptor or the method can be utilized as a discovery approach to identify an unknown receptor to a given ligand. An AlphaScreen experiment can be set up to assess the ligand binding affinity to a given receptor.
- 高通量蛋白纯化
- 人白细胞分化抗原
- 自然自动免疫(natural active immunity)
- 免疫器官(immune organ)
- The Use of Adjuvants in Experimental Vaccines: I. Aluminum Hydroxide Gels
- Methods for In Vitro Generation of Human Type 1 Regulatory T Cells
- Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli
- Interleukin-2 and the Brain: Dissecting Central Versus Peripheral Contributions Using Unique Mouse Models
- Experimental Autoimmune Encephalomyelitis
- Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs