IgG Purification
Several immunological procedures can be successfully carried out using nonpurified antibodies, such as unfractionated antisera or ascitic fluid/culture supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin (Ig) is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity columns cannot usually be efficiently performed with nonpurified immunoglobulin; other procedures may yield artifactual results if whole antiserum or ascitic fluid is used as a source of antibody. Purification of immunoglobulin is therefore at least useful and sometimes essential for a range of immunological methods. This process may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant or the isolation of a particular antigen-binding fraction of Ig from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some type of affinity purification.
- 免疫球蛋白提取 (Immunoglobulin isolation):蛋白质的浓缩技术
- 人丁型肝炎IgM(HDV IgM)酶联免疫分析(ELISA)
- 人二肽基肽酶IV(DPP-4)ELISA试剂盒 说明书
- 人纤溶酶-抗纤溶酶复合物(PAP)ELISA试剂盒 说明书
- 细菌的生理、生化试验简介
- 单克隆抗体的纯化
- 阻断针对移植物(或宿主)的特异性免疫应答
- Something Happens; A Brief History of TCR Signal Transduction
- Purification Of Anti Peptide Antibody
- Investigating the Involvement of Host Factors Involved in Intracellular Pathogen Infection by RNAi in Drosophila Cells