Bivalent Diabodies
关键词: bivalent diabodies来源: 互联网
Single-chain Fv fragments exhibiting monovalent binding can be converted into bivalent molecules by reducing the linker connecting the two variable domains to approximately five residues resulting in homodimerization of two polypeptide chains. This protocol describes the cloning steps to convert an scFv into a diabody molecule and provides some information for production and analysis.
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