Biotin conjugation
实验概要
The protocol describes the conjugation of Biotin to an antibody.
实验步骤
1. ake LCB-NHS (long-chain biotin NHS ester) solution with DMSO (6 mg/ml, 6.5 mM).
2. Add 10 µl of LCB-NHS into 1 ml of 2 mg/ml Ab and incubate at RT for 50 min.
3. Terminate the reaction by adding 0.5 ml of 1 M Tris.HCl, pH 8.0.
4. Dialyse 24-48 hours against 1X PBS.
5. Add 0.1% (w/v) NaN3 if necessary.
This protocol can also be used for any other peptides and proteins.
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